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OPERATING HOURS

Mon-Thu : 9am to 6pm
Fri : 9am to 5.30pm

EQUIPMENT

We have the following laser equipment:

1. Olympus Fluoview FV300
2. Olympus Fluoview FV1000 (At level 4, #04-04)
3. Olympus Fluoview FV1000 (At level 4, #04-25)
4. Carl Zeiss Lsm510 META

Olympus Fluoview FV300

Laser equiped:

10 mW Argon ion 488nm

1mW HeNe Green 543nm

5mW HeNe Red 633nm

A Ti:Sa femto-second laser, the 705 - 980nm Chameleon XR for 2-photon imaging has been installed and undergoing calibration.

Others:

Live cell imaging chamber with 5% CO 2 with air and stage heater.

Olympus Fluoview FV1000 (At level 4, #04-04)

Laser equiped:

20mW solid state 405nm

25 mW Argon ion 458nm, 488nm, 515 nm

1mW HeNe Green 543nm

Others:

Live cell imaging chamber with 5% CO 2 with air and stage heater.

Olympus Fluoview FV1000 (At level 4, #04-25)

Laser equiped:

20mW solid state 405nm

25 mW Argon ion 458nm, 488nm, 515 nm

1mW HeNe Green 543nm

5mW HeNe Red 633nm

Others:

Live cell imaging chamber with 5% CO 2 with air and stage heater.

Carl Zeiss Lsm510 META

Laser equiped:

25 mW Argon ion 458nm, 488nm, 515 nm

1mW HeNe Green 543nm

Others:

Live cell imaging chamber with 5% CO 2 with air and stage heater.

CCD camera for real color imaging by halogen lamp or epi-fluorescence with mercury lamp.

Objectives

(image obtained from www.zeiss.com)

Olympus:

UPlanFl 4X/ 0.13

UPlanApo 10X/ 0.40 0.17

UPlanApo 20X/ 0.70 0.17

UPlanApo 40X/ 1.00 Oil

UPlanApo 40X/ 0.85 0.11-0.23

UPlanApo 60X/ 1.40 Oil 0.17

UPlanFl 60X/ 1.25 Oil 0.17

PlanApo 60X/ 1.00 WLSM 0.17 (Water lens)

UPlanApo 100X/ 1.35 Oil 0.17

UPlanApo 100X/ 1.35 Oil 0.17

Zeiss:

NeoFluar 10X/ 0.30

NeoFluar 20X/ 0.50

Plan-NeoFluar 40X/ 1.30 Oil T/0.17

Plan-NeoFluar 40X/ 0.75 T/0.17

Plan-Apochromat 63X/ 1.40 Oil Dic T/0.17

C-Apochromat 63X/ 1.2 W Korr T/0.15 ¡ìC 0.17 (water lens)

Image Processing Workstation

There are 2 workstations (Intel Pentium 4) with Image Pro Plus, Photoshop CS2, Image J and various image processing software for image processing, analysis, 3D reconstruction and deconvolution respectively.

Network Attached Storage(NAS)

NAS (network attached storage) is a hard disk array for image storage for confocal users. It was composed of 4 hard disks which can be access through internet. NAS is protected by a firewall router and will be back-uped regularly.