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OPERATING HOURS

Mon-Thu : 9am to 6pm
Fri : 9am to 5.30pm

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DIABETES RESEARCH LABORATORY

MEMBERS

Assoc Prof. LI, GuoDong, M.D., Principal Investigator nmiligd@nus.edu.sg

Mr. LUO, Ruihua, M.B.B.S. nmiluorh@nus.edu.sg

Miss ZHANG, Jiping, M.B.B.S. g0300953@nus.edu.sg

Miss XIE, Fei nmixf@nus.edu.sg

Mr. TEH , Song Hooi, B.S. nmitsh@nus.edu.sg

Miss SHAO, Shiying, M.B.B.S. g0600206@nus.edu.sg

Past:

Ms. WEI, Heng (1997-1999)

Dr. XIONG, Zhuowei (1997-1998)

Ms. SONG, Jie (1998-1999)

Dr. HUO, Jianxin (1998-2004)

Dr. LI, Jingsong (1999-2004)

Dr. TANG Yanxia (2002-2005)

 

RESEARCH AREAS

-Generation of functional insulin-producing cells from progenitor and stem cells: differentiation, gene expression,

-metabolism, glucose sensing and responsiveness, in vivo survival and functionality (transplantation) in diabetic animals.

-Biology/physiology/pathology of islet beta-cells, including signaling mechanisms for insulin secretion, control of beta-cell growth and death, role of G-proteins and GTP in beta-cells, toxicity of inflammatory cytokines to beta-cells.

-Pathogenesis of dysfunctional vascular endothelial cells in diabetes, in particular the effects of high glucose and high fatty acids on nitric production and relevant signal transduction in endothelial cells.

 

FUNDING

  • National Medical Research Council, Singapore
  • Biomedical Research Council, Singapore
  • Juvenile Diabetes Research Foundation, USA
  • Academic Research Fund, National University of Singapore

 

RESEARCH COLLABORATORS

Assoc. Prof. Sai Kiang Lim, Genomic Institute of Singapore

Prof. Anjan Kowluru, Department of Pharmaceutical Sciences, and WSU-Comprehensive Diabetes Center, Wayne State University-Detroit, MI 48201, USA

Prof. Paolo Meda, Department of Cell Physiology and Metabolism, University of Geneva, 1211 Genève 4, Switzerland

Assoc. Prof. Oi Lian Kon, National Cancer Center of Singapore

Assoc. Prof. Manuel Salto-Tellez, Department of Pathology, National University of Singapore

 

AWARDS

-“Novel & Newsworthy Top Ten in Cell Biology in 2005" by the American Society for Cell Biology (ASCB) on the work ‘Generation and Verification of Functional Insulin-producing Cells Derived from Embryonic Progenitor Cells’.
http://www.ascb.org/files/2005pressbook.pdf

-Short list of “Best Poster in Basic Research" by Combined Scientific Meeting 2005, Singapore on the work ‘Generation and characterization of insulin-producing cells derived from mouse embryonic progenitor Cells’.

 

PUBLICATION

Peer-reviewed full papers:

CONFERENCE PAPERS

  • 1.       Li GD, Luo R, ZHANG J, Kon OL, Salto-Tellez M, Xie F, and Lim SK. (2006) Insulin-producing Cells Derived from Embryonic Progenitor Cells Reverse Hyperglycemia in Streptozotocin-induced Diabetic Animals. Diabetes 55(suppl. 1): A22 http://scientificsessions.diabetes.org/Abstracts/index.cfm?fuseaction=
    Locator.SearchAbstracts
  • 2.       Tang, YX. and Li, G.D. (2006) High Fatty Acids affect Nitric Oxide Production and Signaling Pathway of the Receptor Agonist Bradykinin in Endothelial Cells. Diabetes 55(suppl. 1): A159
    http://scientificsessions.diabetes.org/Abstracts/index.cfm?fuseaction=
    Locator.SearchAbstracts
  • 3.       Li, G.D Luo, RH. Zhang, J. Kon OL, Salto-Tellez M, Xie, F. Meda P. and Lim, S.K. (2006) Generation of insulin-producing cells from embryonic progenitor cells for transplantation in type 1 diabetic mice. In: Poster Session Abstracts book, p191. Accepted for presentation at the 4th ISSCR Annual Meeting, 29 June - 1 July, 2006, Toronto, Ontario, Canada
    http://www.abstractonline.com/viewer/SearchResults.asp
  • 4.       Zhang, J. Luo, RH. Kon, OL. Xie, F. Lim, S.K. and Li, G.D. (2005) Generation and verification of functional insulin-producing cells derived from embryonic progenitor cells. Mol Biol Cell 16(suppl.): 386a
    http://www.ascb.org/index.cfm?navid=116
  • 5.       Zhang, J. Luo, RH. Kon, OL. Xie, F. Lim, S.K. and Li, G.D. (2005) Generation and characterization of insulin-producing cells derived from mouse embryonic progenitor Cells. Ann Acad Med Sing 34(suppl): S213, Basic Science Poster Award at Combined Scientific Meeting 2005, Singapore
  • 6.       Li, G.D Luo, RH. Xie, F. Zhang, J. and Lim, S.K. (2005) Long term propagation and functionality of insulin-producing cells derived from mouse embryos. Accepted for presentation at the 3rd ISSCR Annual Meeting, 23-25 June 2005, San Francisco, CA, USA
  • 7.       Li, J., Zhang, J. and Li, GD. (2005) Glucose and forskolin induced translocation of phosphatidylinositol 4-phosphate 5-kinase in insulin-secreting INS-1 cells is coupled with Rac1 activation. Diabetes 54(suppl. 1): A421 http://scientificsessions.diabetes.org/Abstracts/index.cfm?fuseaction=
    Locator.SearchAbstracts
  • 8.       Li, G.D Luo, RH. Zhang, J. Xie, F. and Lim, S.K. (2005) Insulin-producing Cells Derived from Mouse Embryo Exhibit Functional Secretory Responses to Secretagogues. Diabetes 54(suppl. 1): Accepted but withdrawn
  • 9.       Li, G.D. and Huo, J. (2004) Induction of apoptosis of insulin-secreting cells by tumor-necrosis-factor-alpha plus interferon-gamma mainly by nitric oxide independent pathways. Mol Biol Cell 15(suppl.): 375a
  • 10.   Li, G.D. Luo, RH. and Lim, S.K. (2004) Generation of functional insulin-producing cells from mouse embryo-derived progenitor cells. Diabetes 53(suppl. 2): A381 (Accepted but withdrawn)
  • 11.   Tang, YX. and Li, G.D. (2004) Chronic exposure to high fatty acids impairs receptor agonist-stimulated nitric oxide production and increments of cytosolic Ca2+ levels in endothelial cells mainly due to activation of protein kinase C. Diabetes 53(suppl. 2): A197 http://scientificsessions.diabetes.org/Abstracts/index.cfm?fuseaction=
    Locator.SearchAbstracts
  • 12.   Li, G.D. and Huo, J. (2003) p-53-independent induction of p21Waf1/Cip1 contributes to the activation of caspases in GTP-depletion induced apoptosis of insulin-secreting cells. Mol Biol Cell 14(suppl.): 159a
  • 13.   Li, J., Huo, J. Luo, RH. Kowluru, A. and Li, G.D. (2003) Involvement of Rac1, a Small G-Protein, in Islet beta-Cell Growth and Insulin Secretion. Diabetes 52(suppl. 1): A372
    http://scientificsessions.diabetes.org/Abstracts/ index.cfm?fuseaction=
    Locator.SearchAbstracts
  • 14.   Tang, YX. and Li, G.D. (2003) Prolonged culture with high glucose causes a reduction in the number of bradykinin receptor in endothelial cells via activation of protein kinase C-beta. Diabetes 52(suppl. 1): A168
    http://scientificsessions.diabetes.org/Abstracts/index.cfm?fuseaction=
    Locator.SearchAbstracts
  • 15.   Amin, R., Chen, H.Q., Li, J., Li, G.D. and Kowluru, A. (2003) Novel roles for Rac in mastoparan-induced insulin secretion. Diabetes 52(suppl. 1): A370 http://scientificsessions.diabetes.org/Abstracts/index.cfm?fuseaction=
    Locator.SearchAbstracts
  • 16.   Li, J., Huo, J. Luo, RH. and Li, G.D. (2002) Role of the G-protein Rac1 in cell growth and insulin secretion in islet (INS-1) beta-cells. 2002 Islet Biology Symposium, 25-28 October, North Falmouth, MA
  • 17.   Li J, Luo R, and Li GD. (2002) Expression of a secretory granule associated protein (syncollin) affects regulated insulin secretion in INS-1 cells. Diabetes, 51(suppl. 2):A596
  • 18.   Tang, YX. and Li, G.D. (2002) Activation of protein kinase C possibly mediates the defected Ca2+ homeostasis in endothelial cells in prolonged high glucose culture. Diabetologia 45 (suppl. 2), A57
  • 19.   Li, G.D. (2002) Biology of the pancreatic islet beta-cell and the consequence of its dysfunction. In: Abstract book of Karolinska Institute-NUS Life Science Symposium, 9-10 February 2002, Singapore. p. 16
  • 20.   Li, J., Luo, RH. and Li, G.D. (2001) Involvement of the small G-protein Rac1 in glucose and forskolin induced insulin secretion in islet (INS-1) beta-cells. Diabetologia 44 (suppl. 1), A62
  • 21.   Huo, J. Metz, SA. and Li, G.D. (2001) A p53-independent p21Waf1/Cip1 pathway probably mediates activation of caspases in GTP-depletion induced apoptosis of insulin-secreting cells. Diabetes 50 (suppl. 2), A348
  • 22.   Li, G.D. Song, J. and Tang, YX. (2001) High fatty acids promote cell growth and affect cytosolic Ca2+ homeostasis in endothelial cells. Diabetologia 44 (suppl. 1), A11
  • 23.   Huo, J. Luo, RH. and Li, G.D. (2001) Activation of caspases by long-term treatment of high free fatty acids in insulin-secreting beta-cells. Diabetes 50 (suppl. 2), A143
  • 24.   Tang, YX. and Li, G.D. (2001) High glucose impairs bradykinin-induced nitric oxide production in endothelial cells by reduction of cytosolic Ca2+ levels. Diabetes 50 (suppl. 2), A41
  • 25.   Li, G.D. and Luo, RH. (2001) Effects of long-term culture of fatty acids on ion fluxes and insulin secretion in islet beta-cells. Diabetes 50 (suppl. 2), A352
  • 26.   Li, GD., Luo, RH., Gao, R. and Gopalarishnakone, P. (2000) Snake venom phospholipase A2 stimulates insulin secretion by increasing cytosolic Ca2+ levels but independent of its enzymatic activity. In: Abstract Handbook of 4th NUH-Faculty of Medicine Annual Scientific Meeting, 30 June & 1 July 2000, Singapore. p.54
  • 27.   Huo, J., Metz, SA. and Li, GD. (2000) Induction of caspase-2 mediated apoptosis of insulin-secreting cells due to GTP depletion. Diabetes 49 (suppl. 1), A259
  • 28.   Li, GD., Luo, RH., Gao, R. and Gopalarishnakone, P. (2000) Stimulation of insulin secretion by snake phospholipase A2 by increasing cytosolic Ca2+ levels but independent of its enzymatic activity. Diabetes 49 (suppl. 1), A252
  • 29.   Li, JS., Luo, RH. and Li, GD. (2000) Inhibition of insulin secretion by the inhibitor of protein kinase A, H-89, mainly by a blockage of calcium channels. Diabetes 49 (suppl. 1), A418
  • 30.   Li, GD. (1999) Sustained depletion of GTP induces death of insulin-secreting cells by apoptosis. In: Abstract Handbook of 3rd NUH-Faculty of Medicine Annual Scientific Meeting, 28-29 August 1999, Singapore. p.74
  • 31.   Huo, J. Metz, SA. and Li, G.D. (1999) Increment of tissue transglutaminase activity in apoptosis of insulin-secreting cells induced by sustained GTP depletion. Diabetes 48 (suppl. 1), A248
  • 32.   Wei, H., Luo, RH. and Li, G.D. (1999) Deffects of intracellular Ca2+ homeostatsis in endothelial cells cultured with high glucose. Diabetes 48 (suppl. 1), A471
  • 33.   Li, G.-D. and Metz, S.A. (1998) Depletion of guanine nucleotides induces depolarization of membrane potential in insulin-secreting cells. Diabetes 47 (suppl. 1), A255
  • 34.   Li, G.-D. and Luo, RH. (1998) Inhibition of insulin secretion from pancreatic beta-cells by butanedione monoxime. Diabetologia 41 (suppl. 1), A152
  • 35.   Li, G.-D. and Luo, R.H. (1998) Dual effects of phosphotyrosine phosphatase inhibitors on insulin secretion from pancreatic beta-cells. Diabetes 47 (suppl. 1), A256
  • 36.   Li, G.-D. and Metz, S.A. (1997) Effect of depletion of guanine nucleotides on cytosolic free Ca2+ levels in insulin-secreting cells. Diabetes 46 (suppl. 1), 273A
  • 37.   Kowluru, A., Li, G.-D., Rabaglia, M., Segu, V.B., Aktories, K., Hofmann, F. and Metz, S.A. (1997) Differential regulation of insulin secretion by Rho subfamily of GTP-binding proteins. Diabetes 46 (suppl. 1), 81A
  • 38.   Kowluru, A., Li, G.-D. and Metz, S.A. (1997) Glucose activates the carboxyl methylation of gamma subunits of trimeric G-proteins in pancreatic beta cells. Diabetes 46 (suppl. 1), 214A
  • 39.   Segu, V.B., Li, G.-D., and Metz, S.A. (1997) Use of a soluble tetrazolium compound to assay metabolic activation of intact pancreatic beta cells. Diabetes 46 (suppl. 1), 227A
  • 40.   Kowluru, A. ; Li, G. ; Rabaglia, M. ; Segu, V. ; Aktories, K.; Hofmann, F.; and Metz, S. (1997) Differential roles of the rho subfamily of GTP-binding proteins in glucose- and calcium-induced insulin secretion. Diabetologia, 40 (Suppl. 1), A43
  • 41.   Kowluru, A.; Li, G.; and Metz, S. (1997) Activation by glucose of the carboxyl methylation of gamma subunits of trimeric G-proteins in pancreatic beta cells. Diabetologia, 40 (Suppl. 1), A117
  • 42.   Li, G.-D. and Metz, S. (1996) Long-term depletion of GTP induces death of insulin-secreting cells probably by apoptosis. Diabetes 45 (Suppl. 2), 155A
  • 43.   Kowluru, A., Seavey, S., Li, G.-D.  and Metz, S. (1996) Carboxyl (de)methylation of gamma subunit of trimeric GTP-binding proteins in insulin-secreting cells: possible regulation by glucose. In: Abstracts of the 10th Internat. Congr. of Endocrinology, June 12-15, San Francisco, USA, P. 786
  • 44.   Kowluru, A., Seavey, S., Li, G.-D. Vadakekalam, J. and Metz, S. (1996) Stimulation by glucose of carboxyl methylation of specific GTP-binding proteins in pancreatic beta cells: relevance to insulin secretion. In: Abstracts of the 10th Internat. Congr. of Endocrinology, June 12-15, San Francisco, USA, P. 765
  • 45.   Meredith, M., Li, G.-D. and Metz, S. (1996) GTP depletion impedes calcium-induced insulin secretion from intact beta(HIT-T15 or INS-1) cells. Diabetes 45 (Suppl. 2), 241A
  • 46.   Li, G.-D., Kowluru, A. and Metz, S. (1995) High activity of prenylcysteine carboxyl methyltransferase in the secretory granule fraction in insulin-secreting INS-1 cells. Diabetes, 44 (Suppl. 1),262A
  • 47.   Kowluru, A., Li, G.-D. Seavey, S., Nesher, R. and Metz, S. (1995) Localization of a methylation-demethylation cycle for GTP-binding proteins in insulin-secreting cells. J. Invest. Med. 43 (Suppl. 2), 354A
  • 48.   Li, G.-D., Rungger, E., Just, I., Aktories, K., Jonas, J.-C. and Wollheim, C.B. (1993) Alteration of actin filaments by clostridium botulinum C2 toxin inhibits insulin secretion from HIT-T15 cells. Diabetologia 36, (Suppl. l) A33
  • 49.   Wollheim, C.B., Li, G.-D. and Regazzi R. (1992) Possible role of small G-proteins in insulin secretion. In: Abstr. of Semmelweis Symposium 1992 "Signal Transduction in Mammalian cels". October 3-5, Budapest, Hungary, p.55-57
  • 50.   Li, G.-D., Regazzi, R., Balch, W.E. and Wollheim, C.B. (1992) An effector domain peptide of GTP-binding protein rab3 stimulates insulin release from permeabilized HIT cells. Diabetologia 35 (Suppl. 1), A12
  • 51.   Rutter, G.A., Li, G.-D., Pralong, W-F. and Wollheim, C.B. (1992) Altered secretory responses of INS-1 and RINm5F cells are associated with differences in InsP3 receptor function. Diabetologia 35, (Suppl. l) A99
  • 52.   Li, G.-D., Regazzi, R., and Wollheim, C.B. (1991) Lovastatin attenuates potentiation but not initiation of nutrient-induced insulin release in HIT cells. Diabetologia 34, (Suppl. 2), A24
  • 53.   Janjic, D., Asfari, M., Meda, P., Li, G.-D., Halban, P.A. and Wollheim, C.B. (1991) Characterization of a 2-mercaptoethanol dependent insulin secreting cell line. Diabetologia 34 (Suppl. 2), A92
  • 54.   Li, G.-D., Milani, D., Pralong, W. and Wollheim, C.B. (1991) Extracellular ATP stimulates insulin secretion by Ca2+ dependent and independent mechanisms. Experientia 47, A59
  • 55.   Milani, D., Li, G.-D., Pralong, W. and Wollheim, C.B. (1990) Exogenous ATP induces insulin release in RINm5F cells via Ca2+-dependent and independent pathways. In: Abstracts of the 18th Aharon Katzir-Katchalsky Conference, September 9-14, Chieti, Italy
  • 56.   Li, G.-D., Milani, D., Pralong, W. and Wollheim, C.B. (1990) Extracellular ATP stimulates insulin secretion by Ca2+ dependent and independent mechanisms. Diabetologia 33, Suppl. A101
  • 57.   Dunne, M.J., Li, G.-D., Milani, D., Wollheim, C.B. and Petersen, O.H. (1990) Externally applied ATP closes ATP-regulated potassium channels, increases cytosolic Ca2+ and evokes the secretion of insulin from clonal pancreatic ß-cells. J. Physiol. 430, 124P
  • 58.   Li, G.-D., Pralong, W., Regazzi, R., Ullrich, S. and Wollheim, C.B. (1989) Potentiation of neurohormone-stimulated insulin secretion in protein kinase C deficient cells. Diabelogia 32, 510A
  • 59.   Bullett, M.J., Dunne, M.J., Li, G.-D., Wollheim, C.B. and Petersen, O.H. (1989) Galanin activates ATP-sensitive K+ channels via a pertussis toxin-sensitive G-protein in rat insulinoma cells. J. Physiol. 410, 19P
  • 60.   Li, G.-D., Ullrich, S. and Wollheim, C.B. (1988) Inositol phosphate generation and insulin secretion by vasopressin in RINm5F cells. Diabetologia 31, 514A
  • 61.   Wollheim, C.B., Regazzi, R., Li, G.-D., Peter-Riesch, W., Pralong, W. and Biden, T.J. (1988) The role of calcium in insulin release. In: Abstracts of the 8th Internat. Congr. of Endocrinology, July 17-23, Kyoto, Japan, P. 13

 

VACANCIES

Postgraduate Scholarship

Post-doctoral Fellow

Research Assistant

Contact: Prof. Li at nmiligd@nus.edu.sg

 

    
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